These Applications Notes are offered for guidance only. They have been supplied to us by DB1 users. However, we can offer no guarantees of their effectiveness. Biotronics Ltd accepts no liabilities for losses incurred by use of these methodologies.
Testicular tissue freezing
Human Sperm Preservation Medium (HSPM)
| COMPONENT | g/l |
| NaCl | 5.844 |
| KCl | 0.400 |
| CaCl2.2H2O | 0.400 |
| MgCl2.6H2O | 0.100 |
| Na lactate | 1.88 ml |
| NaH2PO4.2H2O | 0.050 |
| NaHCO3 | 2.600 |
| HEPES free acid | 4.766 |
| Sucrose | 17.120 |
| Glycine | 10.000 |
| Glucose | 1.000 |
| Gentamycin | 0.005 |
| Glycerol | 150.00 ml |
| HSA (V, powder) | 4.900 |
Standard straws and ministraws, HSPM cryoprotectant;
| ramp | start temp | rate | finish |
| 1 | Ambient | 0.5/min | 5ºC |
| 2 | 5ºC | 0.8ºC/min | 0ºC |
| 3 | 0ºC | Freefall | -60ºC |
| 4 | -60ºC | plunge into LN2 |
