Biotronics Ltd, Unit 1, Moreton Farm, Moreton Eye, Leominster, HR6 0DP
Tel:+44 (0)1568 612402 Fax:+44 (0)1568 616088 Email:Sales@biotronics.net
 

Specimen

Slices or biopsies of ovarian cortical tissue.

Freeze Solution

OV FRZ#1 – 1.5 M DMSO

2ml DMSO + 18ml cryo buffer (SIVF cryo buffer from Cook IVF)

Procedure
    1. If large pieces of tissue have been received, use sterile fine nose scissors or a scalpel blade to cut tissue into 6mm x 1mm pieces.
    2. Transfer one or more pieces of tissue into each straw. Hold on ice for 10 min to ensure cryoprotectant permeation.
    3. At the end of the freeze program, plunge the straws into liquid nitrogen.

Programme

  1. 4 oC to -7oC at -3o/min
  2. Hold 5 min
  3. Seed
  4. Hold 5 min
  5. -7o to -40oC at -0.3o/min
  6. Freefall to -60oC
  7. END
  8. Plunge into liquid nitrogen.

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